We are so glad to annouce our communication to Chem. Sci. in collaboration with our CiQUS colleagues at J. Montenegro's lab, entitled "Supramolecular Caging for Cytosolic Delivery of Anionic Probes" and authored by H. Fernández-Caro, I. Lostalé-Seijo, M. Martinez-Calvo, J. Mosquera, J. L. Mascareñas and J. Montenegro, has been accepted and it's already available through the journal website.
Abstract: The cytosolic delivery of hydrophilic, anionic molecular probes and therapeutics is a major challenge in chemical biology and medicine. Herein, we describe the design and synthesis of peptide-cage hybrids that allow an efficient supramolecular binding, cell membrane translocation and cytosolic delivery of a number of anionic dyes, including pyranine, carboxyfluorescein and several sulfonate-containing Alexa dyes. This supramolecular caging strategy is successful in different cell lines, and the dynamic carrier mechanism has been validated by U-Tube experiments. The high efficiency of the reported approach allowed intracellular pH tracking by exploiting the ratiometric excitation of the pyranine fluorescent probe