Our last paper at Angew. Chem. Int. Ed. is available as accepted article

03/11/2020

We are very happy to share here that our research article at Angew. Chem. Int. Ed., entitled "Intracellular reactions promoted by bis‐histidine miniproteins stapled with Pd(II) complexes" and authored by J. L. Mascarenas, S. Learte-Aymamí, C. Vidal and A. Gutiérrez-González, has been accepted and it's already available through the journal website.

Abstract: The generation of catalytically active metalloproteins inside living mammalian cells is a major research challenge at the interface between catalysis and cell biology. Herein we demonstrate that basic domains of bZIP transcription factors, mutated to include two histidine residues at i, i+4 positions, react with palladium (II) sources to generate catalytically active, stapled pallado‐miniproteins. The resulting constrained peptides are efficiently internalized into living mammalian cells, where they can perform palladium‐promoted depropargylation reactions, without cellular fixation. Control experiments confirm the requirement of the peptide scaffolding and the palladium staple for attaining the intracellular reactivity.



External link: https://onlinelibrary.wiley.com/doi/10.1002/anie.202002032