So happy to share our last collaboration with Mollica's group is already published in the Bioorganic Chemistry journal
We are very glad to announce here that our research article at Bioorg. Chem. in collaboration with Mollica's group, entitled "A novel β-hairpin peptide derived from the ARC repressor selectively interacts with the major groove of B-DNA" and authored by A. Stefanucci, J. Amato, D. Brancaccio, B. Pagano, A. Randazzo, F.
Santoro, L. Mayol, S. Learte, J. Rodríguez, J. L. Mascareñas, E.
Novellino, A. Carotenuto and A. Mollica, has been accepted and it's already available through the journal website.
Abstract: Transcription factors (TFs) have a remarkable role in the homeostasis of
the organisms and there is a growing interest in how they recognize and
interact with specific DNA sequences. TFs recognize DNA using a variety
of structural motifs. Among those, the ribbon-helix-helix (RHH)
proteins, exemplified by the MetJ and ARC repressors, form dimers that
insert antiparallel β-sheets into the major groove of DNA. A great
chemical challenge consists of using the principles of DNA recognition
by TFs to design minimized peptides that maintain the DNA affinity and
specificity characteristics of the natural counterparts. In this
context, a peptide mimic of an antiparallel β-sheet is very attractive
since it can be obtained by a single peptide chain folding in a
β-hairpin structure and can be as short as 14 amino acids or less.
Herein, we designed eight linear and two cyclic dodeca-peptides endowed
with β-hairpins. Their DNA binding properties have been investigated
using fluorescence spectroscopy together with the conformational
analysis through circular dichroism and solution NMR. We found that one
of our peptides, peptide 6, is able to bind DNA, albeit
without sequence selectivity. Notably, it shows a topological
selectivity for the major groove of the DNA which is the interaction
site of ARC and many other DNA-binding proteins. Moreover, we found that
a type I’ β-hairpin folding pattern is a favorite peptide structure for
interaction with the B-DNA major groove. Peptide 6 is a valuable lead compound for the development of novel analogs with sequence selectivity.
External link: https://www.sciencedirect.com/science/article/abs/pii/S0045206821002133